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KMID : 0903520050480020173
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2005 Volume.48 No. 2 p.173 ~ p.177
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Screening of Biological Activities of the Extracts from Basil (Ocimum basilicum L.)
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Kim Jeung-Hoan
Yoon So-Jung
Lee Kyoung-Hwan
Kwon Hyo-Jung
Chun Sung-Sook
Kim Tae-Wan
Cho Young-Je
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Abstract
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Physiological functionalities of water and ethanol extracts from Basil were determined. The concentration of total phenolic compounds of the water and ethanol extracts were 286.0 μg/ml, 250.0 μg/ ml. Antioxidant activities of Basil extracts were determined using 2,2’-azinobis (3-ethyl benzothiazoline- 6-sulfonic acid) radical cations (ABTS), 2,2-diphenyl-1-picryl hydrazyl radicals (DPPH), antioxidant protection factor and thiobarbituric acid reactive substances. The total antioxidant activities of Basil extracts using ABTS were 96.8% in the water extracts and 94.7% in the ethanol extract, DPPH were 87.0%, 93.9%, PF were 0.69, 1.16 and TBARS were 0.2×10?3 μM, 0.6×10?3 μM. Angiotensin converting enzyme inhibitory activity and xanthine oxidase inhibitory activity of Basil were higher in ethanol extracts (99.7%, 100.0%) than those of water extracts (39.9%, 54.7%). Phenolic profiles in Basil extracts were analyzed using HPLC. The result was that among the 6 phenolics, rosemarinic acid was the highest in ethanol extracts.
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KEYWORD
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biological activity, Basil (Ocimum basilicum L), xanthine oxidase, angiotensin converting enzyme, antioxidant
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